H(+)/Cl(‑) exchange transporter 7 promotes lysosomal acidification‑mediated autophagy in mouse cardiomyocytes.

ABSTRACT: Autophagy protects cardiomyocytes in various pathological and physiological conditions; however, the molecular mechanisms underlying its influence and the promotion of autophagic clearance are not completely understood. The present study aimed to explore the role of H(+)/Cl(‑) exchange transporter 7 (CLC‑7) in cardiomyocyte autophagy. In this study, rapamycin was used to induce autophagy in mouse cardiomyocytes, and the changes in CLC‑7 were investigated. The expression levels of CLC‑7 and autophagy‑related proteins, such as microtubule associated protein 1 light chain 3, autophagy related 5 and Beclin 1, were detected using western blotting or immunofluorescence. Autolysosomes were observed and analyzed using transmission electron microscopy and immunofluorescence following CLC‑7 silencin

SUBMITTER: Lin J 

PROVIDER: S-EPMC7845584 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature