Examining the utility of DNA barcodes for the identification of tallgrass prairie flora.

ABSTRACT:

Premise

The tallgrass prairies of North America are one of the most threatened ecosystems in the world, making efficient species identification essential for understanding and managing diversity. Here, we assess DNA barcoding with high-throughput sequencing as a method for rapid plant species identification.

Methods

Using herbarium collections representing the tallgrass prairie flora of Oak Lake Field Station, South Dakota, USA, we amplified and examined four common nuclear and plastid barcode regions (ITS, matK, psbA-trnH, and rbcL), individually and in combination, to test their success in identifying samples to family, genus, and species levels using BLAST searches of three databases of varying size.

Results

Concatenated barcodes increased perf

SUBMITTER: Herzog SA 

PROVIDER: S-EPMC7845766 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature