High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate.

ABSTRACT: Liquid-liquid phase separation (LLPS) is a mechanism of intracellular organization that underlies the assembly of a variety of RNP granules. Fundamental biophysical principles governing LLPS during granule assembly have been revealed by simple in vitro systems, but these systems have limitations when studying the biology of complex, multicomponent RNP granules. Visualization of RNP granules in cells has validated key principles revealed by simple in vitro systems, but this approach presents difficulties for interrogating biophysical features of RNP granules and provides limited ability to manipulate protein, nucleic acid, or small molecule concentrations. Here, we introduce a system that builds upon recent insights into the mechanisms underlying RNP granule assembly and permits high-fideli

SUBMITTER: Freibaum BD 

PROVIDER: S-EPMC7845923 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature