Genome-wide prediction of topoisomerase IIβ binding by architectural factors and chromatin accessibility.

ABSTRACT: DNA topoisomerase II-β (TOP2B) is fundamental to remove topological problems linked to DNA metabolism and 3D chromatin architecture, but its cut-and-reseal catalytic mechanism can accidentally cause DNA double-strand breaks (DSBs) that can seriously compromise genome integrity. Understanding the factors that determine the genome-wide distribution of TOP2B is therefore not only essential for a complete knowledge of genome dynamics and organization, but also for the implications of TOP2-induced DSBs in the origin of oncogenic translocations and other types of chromosomal rearrangements. Here, we conduct a machine-learning approach for the prediction of TOP2B binding using publicly available sequencing data. We achieve highly accurate predictions, with accessible chromatin and architectural f

SUBMITTER: Martinez-Garcia PM 

PROVIDER: S-EPMC7845959 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature