The Proteomic Landscape of Centromeric Chromatin Reveals an Essential Role for the Ctf19^CCAN Complex in Meiotic Kinetochore Assembly.

ABSTRACT: Kinetochores direct chromosome segregation in mitosis and meiosis. Faithful gamete formation through meiosis requires that kinetochores take on new functions that impact homolog pairing, recombination, and the orientation of kinetochore attachment to microtubules in meiosis I. Using an unbiased proteomics pipeline, we determined the composition of centromeric chromatin and kinetochores at distinct cell-cycle stages, revealing extensive reorganization of kinetochores during meiosis. The data uncover a network of meiotic chromosome axis and recombination proteins that bind to centromeres in the absence of the microtubule-binding outer kinetochore sub-complexes during meiotic prophase. We show that the Ctf19c^CCAN inner kinetochore complex is essential for kinetochore organization i

SUBMITTER: Borek WE 

PROVIDER: S-EPMC7846277 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature