An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions.

ABSTRACT: Ex vivo characterisation of arterial biomechanics enables detailed discrimination of the various cellular and extracellular contributions to arterial stiffness. However, ex vivo biomechanical studies are commonly performed under quasi-static conditions, whereas dynamic biomechanical behaviour (as relevant in vivo) may differ substantially. Hence, we aim to (1) develop an integrated set-up for quasi-static and dynamic biaxial biomechanical testing, (2) quantify set-up reproducibility, and (3) illustrate the differences in measured arterial stiffness between quasi-static and dynamic conditions. Twenty-two mouse carotid arteries were mounted between glass micropipettes and kept fully vasodilated. While recording pressure, axial force (F), and inner diameter, arteries were exposed to (1) quasi

SUBMITTER: van der Bruggen MM 

PROVIDER: S-EPMC7846753 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature