Single cell sequencing reveals endothelial plasticity with transient mesenchymal activation after myocardial infarction.

ABSTRACT: Endothelial cells play a critical role in the adaptation of tissues to injury. Tissue ischemia induced by infarction leads to profound changes in endothelial cell functions and can induce transition to a mesenchymal state. Here we explore the kinetics and individual cellular responses of endothelial cells after myocardial infarction by using single cell RNA sequencing. This study demonstrates a time dependent switch in endothelial cell proliferation and inflammation associated with transient changes in metabolic gene signatures. Trajectory analysis reveals that the majority of endothelial cells 3 to 7 days after myocardial infarction acquire a transient state, characterized by mesenchymal gene expression, which returns to baseline 14 days after injury. Lineage tracing, using the Cdh5-CreER

SUBMITTER: Tombor LS 

PROVIDER: S-EPMC7846794 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature