Splicing-associated chromatin signatures: a combinatorial and position-dependent role for histone marks in splicing definition.

ABSTRACT: Alternative splicing relies on the combinatorial recruitment of splicing regulators to specific RNA binding sites. Chromatin has been shown to impact this recruitment. However, a limited number of histone marks have been studied at a global level. In this work, a machine learning approach, applied to extensive epigenomics datasets in human H1 embryonic stem cells and IMR90 foetal fibroblasts, has identified eleven chromatin modifications that differentially mark alternatively spliced exons depending on the level of exon inclusion. These marks act in a combinatorial and position-dependent way, creating characteristic splicing-associated chromatin signatures (SACS). In support of a functional role for SACS in coordinating splicing regulation, changes in the alternative splicing of SACS-marke

SUBMITTER: Agirre E 

PROVIDER: S-EPMC7846797 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature