Bioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris.

ABSTRACT: Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity. As a result, these systems are not easily portable or reusable for on-demand protein production applications. In this study, we employ Bioproduced Proteins On Demand (Bio-POD), a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable, on-demand production and secretion of medium- and high-molecular weight proteins (in this case, SEAP, α-amylase, and anti-HER2). The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and rehydration. The hydrogel platform described here is the first hydrogel immobiliz

SUBMITTER: Yuan SF 

PROVIDER: S-EPMC7846901 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature