Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI.

ABSTRACT:

Background

Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was therefore to spatially detect metabolites and lipids on prostate tissue sections by using mass spectrometry imaging (MSI), a method that facilitates molecular imaging of heterogeneous tissue sections, which can subsequently be related to the histology of the same section.

Methods

Here, we simultaneously obtained metabolic and lipidomic profiles in different prostate tissue types using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MSI. Both positive and negative ion mode were applied to analyze consecutive sections from 45 fresh-frozen hum

SUBMITTER: Andersen MK 

PROVIDER: S-EPMC7847144 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature