High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells.

ABSTRACT: Similar to the recent COVID-19 pandemic, influenza A virus poses a constant threat to the global community. For the treatment of flu disease, both antivirals and vaccines are available with vaccines the most effective and safest approach. In order to overcome limitations in egg-based vaccine manufacturing, cell culture-based processes have been established. While this production method avoids egg-associated risks in face of pandemics, process intensification using animal suspension cells in high cell density perfusion cultures should allow to further increase manufacturing capacities worldwide. In this work, we demonstrate the development of a perfusion process using Madin-Darby canine kidney (MDCK) suspension cells for influenza A (H1N1) virus production from scale-down shake flask cultiv

SUBMITTER: Wu Y 

PROVIDER: S-EPMC7847233 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature