Healthy cells functionally present TAP-independent SSR1 peptides: implications for selection of clinically relevant antigens.

ABSTRACT: Tumors with an impaired transporter associated with antigen processing (TAP) present several endoplasmic reticulum-derived self-antigens on HLA class I (HLA-I) which are absent on healthy cells. Selection of such TAP-independent antigens for T cell-based immunotherapy should include analysis of their expression on healthy cells to prevent therapy-induced adverse toxicities. However, it is unknown how the absence of clinically relevant antigens on healthy cells needs to be validated. Here, we monitored TAP-independent antigen presentation on various healthy cells after establishing a T cell tool recognizing a TAP-independent signal sequence receptor 1-derived antigen. We found that most but not all healthy cells present this antigen under normal and inflammatory conditions, indicating that

SUBMITTER: de Waard AA 

PROVIDER: S-EPMC7847959 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature