TransSynW: A single-cell RNA-sequencing based web application to guide cell conversion experiments.

ABSTRACT: Generation of desired cell types by cell conversion remains a challenge. In particular, derivation of novel cell subtypes identified by single-cell technologies will open up new strategies for cell therapies. The recent increase in the generation of single-cell RNA-sequencing (scRNA-seq) data and the concomitant increase in the interest expressed by researchers in generating a wide range of functional cells prompted us to develop a computational tool for tackling this challenge. Here we introduce a web application, TransSynW, which uses scRNA-seq data for predicting cell conversion transcription factors (TFs) for user-specified cell populations. TransSynW prioritizes pioneer factors among predicted conversion TFs to facilitate chromatin opening often required for cell conversion. In additi

SUBMITTER: Ribeiro MM 

PROVIDER: S-EPMC7848352 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature