Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II.

ABSTRACT: Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study, oxidative modifications of the D1 and D2 proteins by the superoxide anion (O₂^•-) and the hydroxyl (HO^•) radicals were studied in WT and a tocopherol cyclase (vte1) mutant, which is deficient in the lipid-soluble antioxidant α-tocopherol. In the absence of this antioxidant, high-resolution tandem mass spectrometry was used to identify oxidation of D1:¹³⁰E to hydroxyglutamic acid by O₂^•- at the Pheo_D

SUBMITTER: Kumar A 

PROVIDER: S-EPMC7848471 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature