Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels.

ABSTRACT: Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibility, matrix remodeling, and biological integration. Despite these advantages, natural protein-based gels have lagged behind synthetic alternatives in their tunability; methods to selectively modulate the biochemical properties of these networks in a user-defined and heterogeneous fashion that can drive encapsulated cell function have not yet been established. Here, we report a generalizable strategy utilizing a photomediated oxime ligation to covalently decorate naturally derived hydrogels with

SUBMITTER: Batalov I 

PROVIDER: S-EPMC7848611 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature