Quantification and gene expression of Lactobacillus casei group species associated with dentinal lesions in early childhood caries.

ABSTRACT:

Background

Considering that the Lactobacillus casei group is strongly associated with caries progression, the use of lactobacilli as probiotics must be balanced due to their possible involvement in dental caries.

Objective

This study aimed to detect and quantify L. paracasei, L. rhamnosus, and L. casei group species in the active and arrested dentinal lesions of preschoolers. It also aimed to determine the expression profiles of lactobacilli genes related to adhesion, extracellular polymeric substance regulation, and pyruvate oxidation.

Methods

Total ribonucleic acid (RNA) was extracted from dentinal lesion samples (25 active, 13 arrested) of children between 2 and 5 years of age. The samples were converted to complementary deoxyribonucleic

SUBMITTER: Reis ACM 

PROVIDER: S-EPMC7848803 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature