Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level.

ABSTRACT: Iron-reducing microorganisms (FeRM) play key roles in many natural and engineering processes. Visualizing and isolating FeRM from multispecies samples are essential to understand the in situ location and geochemical role of FeRM. Here, we visualized FeRM by a "turn-on" Fe²⁺-specific fluorescent chemodosimeter (FSFC) with high sensitivity, selectivity, and stability. This FSFC could selectively identify and locate active FeRM from either pure culture, coculture of different bacteria, or sediment-containing samples. Fluorescent intensity of the FSFC could be used as an indicator of Fe²⁺ concentration in bacterial cultures. By combining the use of the FSFC with that of a single-cell sorter, we obtained three FSFC-labeled cells from an enriched consortium, and all

SUBMITTER: Gan C 

PROVIDER: S-EPMC7848898 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature