Distinct profile of CD34⁺ cells and plasma-derived extracellular vesicles from triple-negative patients with Myelofibrosis reveals potential markers of aggressive disease.

ABSTRACT:

Background

Myelofibrosis (MF) is a clonal disorder of hemopoietic stem/progenitor cells (HSPCs) with high prevalence in elderly patients and mutations in three driver genes (JAK2, MPL, or CALR). Around 10-15% of patients are triple-negative (TN) for the three driver mutations and display significantly worse survival. Circulating extracellular vesicles (EVs) play a role in intercellular signaling and are increased in inflammation and cancer. To identify a biomolecular signature of TN patients, we comparatively evaluated the circulating HSPCs and their functional interplay with the microenvironment focusing on EV analysis.

Methods

Peripheral blood was collected from MF patients (n = 29; JAK2^V617F mutation, n = 23; TN, n = 6) and healthy donors (HD, n = 10). Immunoma

SUBMITTER: Forte D 

PROVIDER: S-EPMC7849077 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature