Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.

ABSTRACT:

Background

Our previous study have shown that the PSMD11 protein was an important survival factor for cancer cells except for its key role in regulation of assembly and activity of the 26S proteasome. To further investigate the role of PSMD11 in carcinogenesis, we constructed a conditional exon 5 floxed allele of PSMD11 (PSMD11^flx) in mice.

Results

It was found that homozygous PSMD11 ^flx/flx mice showed normal and exhibited a normal life span and fertility, and showed roughly equivalent expression of PSMD11 in various tissues, suggesting that the floxed allele maintained the wild-type function. Cre recombinase could induce efficient knockout of the floxed PSMD11 allele both in vitro and in vivo. Mice with constitutive single allele deletion of PSMD11 de

SUBMITTER: Zhao L 

PROVIDER: S-EPMC7849139 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature