Endometrial extracellular matrix rigidity and IFNτ ensure the establishment of early pregnancy through activation of YAP.

ABSTRACT:

Background

In mammals, early pregnancy is a critical vulnerable period during which complications may arise, including pregnancy failure. Establishment of a maternal endometrial acceptance phenotype is a prerequisite for semiheterogeneous embryo implantation, comprising the rate-limiting step of early pregnancy.

Methods

Confocal fluorescence, immunohistochemistry and western blot for nuclear and cytoplasmic protein were used to examine the activation of yes-associated protein (YAP) in uterine tissue and primary endometrial cells. The target binding between miR16a and YAP was verified by dual-luciferase reporter gene assay. The mouse pregnancy model and pseudopregnancy model were used to investigate the role of YAP in the maternal uterus during early pregnancy in vivo.

Res

SUBMITTER: Zhang T 

PROVIDER: S-EPMC7849163 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature