Dengue diagnostics: serious inaccuracies are likely to occur if pre-analytical conditions are not strictly followed.

ABSTRACT:

Background

The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting.

Objectives

To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue.

Methods

Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions.

Findings

Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and vi

SUBMITTER: Iani FCM 

PROVIDER: S-EPMC7849175 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature