DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolution.

ABSTRACT: DNA single-strand breaks (SSBs), or "nicks," are the most common form of DNA damage. Oxidative stress, endogenous enzyme activities, and other processes cause tens of thousands of nicks per cell per day. Accumulation of nicks, caused by high rates of occurrence or defects in repair enzymes, has been implicated in multiple diseases. However, improved methods for nick analysis are needed to characterize the mechanisms of these processes and learn how the location and number of nicks affect cells, disease progression, and health outcomes. In addition to natural processes, including DNA repair, leading genome editing technologies rely on nuclease activity, including nick generation, at specific target sites. There is currently a pressing need for methods to study off-target nicking activity ge

SUBMITTER: Elacqua JJ 

PROVIDER: S-EPMC7849381 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature