Evolutionary Dynamics of Abundant 7-bp Satellites in the Genome of Drosophila virilis.

ABSTRACT: The factors that drive the rapid changes in abundance of tandem arrays of highly repetitive sequences, known as satellite DNA, are not well understood. Drosophila virilis has one of the highest relative amounts of simple satellites of any organism that has been studied, with an estimated >40% of its genome composed of a few related 7-bp satellites. Here, we use D. virilis as a model to understand technical biases affecting satellite sequencing and the evolutionary processes that drive satellite composition. By analyzing sequencing data from Illumina, PacBio, and Nanopore platforms, we identify platform-specific biases and suggest best practices for accurate characterization of satellites by sequencing. We use comparative genomics and cytogenetics to demonstrate that the highly abundant AAA

SUBMITTER: Flynn JM 

PROVIDER: S-EPMC7849990 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature