NEO100 enables brain delivery of blood‒brain barrier impermeable therapeutics.

ABSTRACT:

Background

Intracarotid injection of mannitol has been applied for decades to support brain entry of therapeutics that otherwise do not effectively cross the blood-brain barrier (BBB). However, the elaborate and high-risk nature of this procedure has kept its use restricted to well-equipped medical centers. We are developing a more straightforward approach to safely open the BBB, based on the intra-arterial (IA) injection of NEO100, a highly purified version of the natural monoterpene perillyl alcohol.

Methods

In vitro barrier permeability with NEO100 was evaluated by transepithelial/transendothelial electrical resistance and antibody diffusion assays. Its mechanism of action was studied by western blot, microarray analysis, and electron microscopy. In mouse models, we perfo

SUBMITTER: Wang W 

PROVIDER: S-EPMC7850137 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature