Scleraxis-lineage cell depletion improves tendon healing and disrupts adult tendon homeostasis.

ABSTRACT: Despite the requirement for Scleraxis-lineage (Scx^Lin) cells during tendon development, the function of Scx^Lin cells during adult tendon repair, post-natal growth, and adult homeostasis have not been defined. Therefore, we inducibly depleted Scx^Lin cells (ScxLin^DTR) prior to tendon injury and repair surgery and hypothesized that ScxLin^DTR mice would exhibit functionally deficient healing compared to wild-type littermates. Surprisingly, depletion of Scx^Lin cells resulted in increased biomechanical properties without impairments in gliding function at 28 days post-repair, indicative of regeneration. RNA sequencing of day 28 post-repair tendons highlighted differences in matrix-related genes, cell motility, cytoskeletal

SUBMITTER: Best KT 

PROVIDER: S-EPMC7850622 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature