Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin.

ABSTRACT: The 3D organization of chromatin regulates many genome functions. Our understanding of 3D genome organization requires tools to directly visualize chromatin conformation in its native context. Here we report an imaging technology for visualizing chromatin organization across multiple scales in single cells with high genomic throughput. First we demonstrate multiplexed imaging of hundreds of genomic loci by sequential hybridization, which allows high-resolution conformation tracing of whole chromosomes. Next we report a multiplexed error-robust fluorescence in situ hybridization (MERFISH)-based method for genome-scale chromatin tracing and demonstrate simultaneous imaging of more than 1,000 genomic loci and nascent transcripts of more than 1,000 genes together with landmark nuclear structur

SUBMITTER: Su JH 

PROVIDER: S-EPMC7851072 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature