Statistical factorial designs for optimum production of thermostable α-amylase by the degradative bacterium Parageobacillus thermoglucosidasius Pharon1 isolated from Sinai, Egypt.

ABSTRACT:

Background

This study aimed to isolate potent thermophilic and amylolytic bacteria from a hot spring of Pharaoh's bath, Sinai, Egypt, and screen its degradative activity. The amylolytic activity was further optimized using a statistical full factorial design followed by response surface methodology.

Results

A thermophilic bacterium was isolated from the hot spring of Pharaoh's Bath, Sinai, Egypt. The isolate produced amylase, cellulase, and caseinase and was identified by 16S rRNA gene sequencing as Parageobacillus thermoglucosidasius Pharon1 (MG965879). A growth medium containing 1% soluble starch was found to optimize the amylase production. Dinitrosalycalic acid method (DNS) was used to estimate the amount of reducing sugar produced. Statistical full factorial and respons

SUBMITTER: Saeed AM 

PROVIDER: S-EPMC7851196 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature