Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing.

ABSTRACT: Necrosis, a form of cell death, occurs not only with the development of various diseases but also with a tumor tissue response to cancer treatment. Therefore, pursuing progress for cancer therapy through induction of necrosis may be one of the most effective approaches for cancer eradication. We herein describe the development of a real-time imaging system to visualize intratumoral necrosis. The system is composed of two types of cells expressing either one of two necrosis imaging reporters that consist of a DnaE intein sequence linking to one of two split-luciferase fragments. When necrosis occurs in a tumor composed of both of the cells, the two types of leaked reporters can reconstitute the enzymatic activity as a result of protein trans-splicing and thereby emit bioluminescence

SUBMITTER: Kagiya G 

PROVIDER: S-EPMC7851486 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature