Proteomics of Herpes Simplex Virus Type 1 Nuclear Capsids.

ABSTRACT: Herpes simplex virus replicates in the nucleus, where new capsids are assembled. It produces procapsids devoid of nucleic acid but containing the preVP22a scaffold protein. These thermo-unstable particles then mature into A-, B- or C-nuclear icosahedral capsids, depending on their ability to shed the proteolytically processed scaffold and incorporation of the viral genome. To study how these viral capsids differ, we performed proteomics studies of highly enriched HSV-1 A-, B- and C-nuclear capsids, relying in part on a novel and powerful flow virometry approach to purify C-capsids. We found that the viral particles contained the expected capsid components and identified several tegument proteins in the C-capsid fraction (pU_L21, pU_L36, pU_L46, pU_L4

SUBMITTER: El Bilali N 

PROVIDER: S-EPMC7851555 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature