Expanding the Enzyme Repertoire for Sugar Nucleotide Epimerization: The CDP-Tyvelose 2-Epimerase from <i>Thermodesulfatator atlanticus</i> for Glucose/Mannose Interconversion.

ABSTRACT: Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we discovered a promiscuous type of CDP-tyvelose 2-epimerase (TyvE)-like enzyme that promotes C2-epimerization in all nucleotide (CDP, UDP, GDP, ADP, TDP)-activated forms of d-glucose. This new epimerase, originating from Thermodesulfatator atlanticus, is a functional homodimer that contains one tightly bound NAD⁺/subunit and shows optimum activity at 70°C and pH 9.5. The enzyme exhibits a k _cat with CDP-dglucose of ∼1.0 min^-1 (pH 7.5,

SUBMITTER: Rapp C 

PROVIDER: S-EPMC7851689 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature