Molecular basis of ice-binding and cryopreservation activities of type III antifreeze proteins.

ABSTRACT: Antifreeze proteins (AFPs) can inhibit the freezing of body fluid at subzero temperatures to promote the survival of various organisms living in polar regions. Type III AFPs are categorized into three subgroups, QAE1, QAE2, and SP isoforms, based on differences in their isoelectric points. We determined the thermal hysteresis (TH), ice recrystallization inhibition (IRI), and cryopreservation activity of three isoforms of the notched-fin eelpout AFP and their mutant constructs and characterized their structural and dynamic features using NMR. The QAE1 isoform is the most active among the three classes of III AFP isoforms, and the mutants of inactive QAE2 and SP isoforms, QAE2^ACT and SP^ACT, displayed the full TH and IRI activities with resepect to QAE1 isoform. Cryopres

SUBMITTER: Choi SR 

PROVIDER: S-EPMC7851773 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature