A lysate proteome engineering strategy for enhancing cell-free metabolite production.

ABSTRACT: Cell-free systems present a significant opportunity to harness the metabolic potential of diverse organisms. Removing the cellular context provides the ability to produce biological products without the need to maintain cell viability and enables metabolic engineers to explore novel chemical transformation systems. Crude extracts maintain much of a cell's capabilities. However, only limited tools are available for engineering the contents of the extracts used for cell-free systems. Thus, our ability to take full advantage of the potential of crude extracts for cell-free metabolic engineering is constrained. Here, we employ Multiplex Automated Genomic Engineering (MAGE) to tag proteins for selective depletion from crude extracts so as to specifically direct chemical production. Specific edi

SUBMITTER: Garcia DC 

PROVIDER: S-EPMC7851839 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature