Functional relationships of three NFU proteins in the biogenesis of chloroplastic iron-sulfur clusters.

ABSTRACT: Iron-sulfur clusters are required in a variety of biological processes. Biogenesis of iron-sulfur clusters includes assembly of iron-sulfur clusters on scaffold complexes and transfer of iron-sulfur clusters to recipient apoproteins by iron-sulfur carriers, such as nitrogen-fixation-subunit-U (NFU)-type proteins. Arabidopsis thaliana has three plastid-targeted NFUs: NFU1, NFU2, and NFU3. We previously discovered that nfu2 ^-/- nfu3 ^-/- mutants are embryo lethal. The lack of viable nfu2 ^-/- nfu3 ^-/- mutants posed a serious challenge. To overcome this problem, we characterized nfu2-1 ^-/- nfu3-2^+/- and nfu2-1^+/- nfu3-2 ^-/- sesquimutants. Simultaneous l

SUBMITTER: Satyanarayan MB 

PROVIDER: S-EPMC7851846 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature