Two high-yield complementary methods to sort cell populations by their 2D or 3D migration speed.

ABSTRACT: The potential to migrate is one of the most fundamental functions for various epithelial, mesenchymal, and immune cells. Image analysis of motile cell populations, both primary and cultured, typically reveals an intercellular variability in migration speeds. However, cell migration chromatography, the sorting of large populations of cells based on their migratory characteristics, cannot be easily performed. The lack of such methods has hindered our understanding of the direct correlation between the capacity to migrate and other cellular properties. Here, we report two novel, easily implementable and readily scalable methods to sort millions of live migratory cancer and immune cells based on their spontaneous migration in two-dimensional and three-dimensional microenvironments, respectivel

SUBMITTER: Arora A 

PROVIDER: S-EPMC7851856 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature