Microtubule-dependent pushing forces contribute to long-distance aster movement and centration in Xenopus laevis egg extracts.

ABSTRACT: During interphase of the eukaryotic cell cycle, the microtubule (MT) cytoskeleton serves as both a supportive scaffold for organelles and an arborized system of tracks for intracellular transport. At the onset of mitosis, the position of the astral MT network, specifically its center, determines the eventual location of the spindle apparatus and ultimately the cytokinetic furrow. Positioning of the MT aster often results in its movement to the center of a cell, even in large blastomeres hundreds of microns in diameter. This translocation requires positioning forces, yet how these forces are generated and then integrated within cells of various sizes and geometries remains an open question. Here we describe a method that combines microfluidics, hydrogels, and Xenopus laevis egg extra

SUBMITTER: Sulerud T 

PROVIDER: S-EPMC7851858 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature