Cellular response to spinal cord injury in regenerative and non-regenerative stages in Xenopus laevis.

ABSTRACT:

Background

The efficient regenerative abilities at larvae stages followed by a non-regenerative response after metamorphosis in froglets makes Xenopus an ideal model organism to understand the cellular responses leading to spinal cord regeneration.

Methods

We compared the cellular response to spinal cord injury between the regenerative and non-regenerative stages of Xenopus laevis. For this analysis, we used electron microscopy, immunofluorescence and histological staining of the extracellular matrix. We generated two transgenic lines: i) the reporter line with the zebrafish GFAP regulatory regions driving the expression of EGFP, and ii) a cell specific inducible ablation line with the same GFAP regulatory regions. In addition, we used FACS to isolate EGFP⁺ cells

SUBMITTER: Edwards-Faret G 

PROVIDER: S-EPMC7852093 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature