Microbial diversity characterization of seawater in a pilot study using Oxford Nanopore Technologies long-read sequencing.

ABSTRACT:

Objective

Currently the majority of non-culturable microbes in sea water are yet to be discovered, Nanopore offers a solution to overcome the challenging tasks to identify the genomes and complex composition of oceanic microbiomes. In this study we evaluate the utility of Oxford Nanopore Technologies (ONT) sequencing to characterize microbial diversity in seawater from multiple locations. We compared the microbial species diversity of retrieved environmental samples from two different locations and time points.

Results

With only three ONT flow cells we were able to identify thousands of organisms, including bacteriophages, from which a large part at species level. It was possible to assemble genomes from environmental samples with Flye. In several cases this resulted in > 1

SUBMITTER: Liem M 

PROVIDER: S-EPMC7852107 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature