A simple Ca²⁺-imaging approach to neural network analyses in cultured neurons.

ABSTRACT:

Background

Ca²⁺-imaging is a powerful tool to measure neuronal dynamics and network activity. To monitor network-level changes in cultured neurons, neuronal activity is often evoked by electrical or optogenetic stimulation and assessed using multi-electrode arrays or sophisticated imaging. Although such approaches allow detailed network analyses, multi-electrode arrays lack single-cell precision, whereas optical physiology generally requires advanced instrumentation that may not be universally available.

New method

Here we developed a simple, stimulation-free protocol with associated Matlab algorithms that enables scalable analyses of spontaneous network activity in cultured human and mouse neurons. The approach allows analysis of the overall network activity and

SUBMITTER: Sun Z 

PROVIDER: S-EPMC7853247 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature