Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2.

ABSTRACT: We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

SUBMITTER: Hogan CA 

PROVIDER: S-EPMC7853532 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature