Yeast Single-cell RNA-seq, Cell by Cell and Step by Step.

ABSTRACT: Single-cell RNA-seq (scRNA-seq) has become an established method for uncovering the intrinsic complexity within populations. Even within seemingly homogenous populations of isogenic yeast cells, there is a high degree of heterogeneity that originates from a compact and pervasively transcribed genome. Research with microorganisms such as yeast represents a major challenge for single-cell transcriptomics, due to their small size, rigid cell wall, and low RNA content per cell. Because of these technical challenges, yeast-specific scRNA-seq methodologies have recently started to appear, each one of them relying on different cell-isolation and library-preparation methods. Consequently, each approach harbors unique strengths and weaknesses that need to be considered. We have recently developed a

SUBMITTER: Nadal-Ribelles M 

PROVIDER: S-EPMC7854150 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature