High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain.

ABSTRACT: Activation of type 1 cannabinoid (CB₁) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB₁ receptors in the brain. In situ hybridization and immunohistochemical techniques have been crucial for defining the CB₁ receptor expression and localization at the cellular level. Nevertheless, more advanced methods are needed to reveal the precise topography of CB₁ receptors in the brain, especially in unsuspected sites such as other cell types and organelles with low receptor expression (e.g., glutamatergic neurons, astrocytes, mitochondria). High-resolution immunoelectron microscopy provides a more precise dete

SUBMITTER: Puente N 

PROVIDER: S-EPMC7854156 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature