Adoptive Transfer of Monocytes Sorted from Bone Marrow.

ABSTRACT: Inflammatory Ly6C^hi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to assess the fate and recruitment of inflammatory Ly6C^hi monocytes in the tumor microenvironment.

SUBMITTER: Laoui D 

PROVIDER: S-EPMC7854204 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature