Tandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors.

ABSTRACT: Identification of protein-protein interactions of bacterial effectors and cellular targets during infection is at the core to understand how bacteria manipulate the infected host to overcome the immune response. Potential interacting proteins might be identified by genetic methods, i.e., two hybrid screens and could be verified by co-immunoprecipitation. The tandem affinity purification (TAP) method allows an unbiased screen of potential interaction partners of bacterial effectors in a physiological approach: target cells can be infected with a bacterial strain harboring the TAP-tagged bacterial effector protein which is translocated in the host similar as under physiological infection conditions. No transfection and overexpression of the bacterial protein in the eukaryotic host are

SUBMITTER: Berneking L 

PROVIDER: S-EPMC7854218 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature