A High-throughput qPCR-based Method to Genotype the SOD1^G93A Mouse Model for Relative Copy Number.

ABSTRACT: The most commonly used mouse model in ALS preclinical research expresses multiple copies of the human SOD1 (G93A) transgene. During the course of breeding, successive generations of mice can lose copies of the transgene. Because shorter lifespan of these mice is dependent on transgene copy number, it is essential to ensure that no low-copy, and therefore longer-lived, mice are included in preclinical studies. Existing techniques for SOD1^G93A mouse genotyping are broadly based on creating a standard curve using a reference gene and deducing the relative amount of SOD1 by comparison with the standard curve. This type of technique is used in Alexander et al. (2004) , Vieira et al. (2017) and Maier et al. (2018) . However, it is not described in detail (see Note

SUBMITTER: Tassinari VR 

PROVIDER: S-EPMC7854241 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature