Linking Virus Discovery to Immune Responses Visualized during Zebrafish Infections.

ABSTRACT: The discovery of new viruses currently outpaces our capacity for experimental examination of infection biology. To better couple virus discovery with immunology, we genetically modified zebrafish to visually report on virus infections. After generating a strain that expresses green fluorescent protein (GFP) under an interferon-stimulated gene promoter, we repeatedly observed transgenic larvae spontaneously expressing GFP days after hatching. RNA sequencing comparisons of co-housed GFP-positive and GFP-negative zebrafish revealed a naturally occurring picornavirus that induced a canonical interferon-mediated response and hundreds of antiviral defense genes not observed following immunostimulatory treatments or experimental infections with other viruses. Among the many genes induced by picor

SUBMITTER: Balla KM 

PROVIDER: S-EPMC7854381 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature