Perivascular Secretome Influences Hematopoietic Stem Cell Maintenance in a Gelatin Hydrogel.

ABSTRACT: Adult hematopoietic stem cells (HSCs) produce the body's full complement of blood and immune cells. They reside in specialized microenvironments, or niches, within the bone marrow. The perivascular niche near blood vessels is believed to help maintain primitive HSCs in an undifferentiated state but demonstration of this effect is difficult. In vivo studies make it challenging to determine the direct effect of the endosteal and perivascular niches as they can be in close proximity, and two-dimensional in vitro cultures often lack an instructive extracellular matrix environment. We describe a tissue engineering approach to develop and characterize a three-dimensional perivascular tissue model to investigate the influence of the perivascular secretome on HSC behavior. We generate 3D endotheli

SUBMITTER: Barnhouse V 

PROVIDER: S-EPMC7854499 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature