Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen.

ABSTRACT: Traction force microscopy (TFM) is an important family of techniques used to measure and study the role of cellular traction forces (CTFs) associated with many biological processes. However, current standard TFM methods rely on imaging techniques that do not provide the experimental capabilities necessary to study CTFs within 3D collective and dynamic systems embedded within optically scattering media. Traction force optical coherence microscopy (TF-OCM) was developed to address these needs, but has only been demonstrated for the study of isolated cells embedded within optically clear media. Here, we present computational 4D-OCM methods that enable the study of dynamic invasion behavior of large tumor spheroids embedded in collagen. Our multi-day, time-lapse imaging data provided detailed

SUBMITTER: Mulligan JA 

PROVIDER: S-EPMC7854660 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature