Validation of the bag-mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya.

ABSTRACT:

Aims

This study compared the bag-mediated filtration system (BMFS) and standard WHO two-phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin-like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016.

Methods and results

Environmental samples were collected in Nairobi, Kenya (Sept 2015-Feb 2017), concentrated via BMFS and two-phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two-phase samples (P < 0·05). In BMFS samples, SL PV detection did not significantly differ with volume filtered, filtration time or filter shipment

SUBMITTER: Fagnant-Sperati CS 

PROVIDER: S-EPMC7854911 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature