Cocaine Triggers Astrocyte-Mediated Synaptogenesis.

ABSTRACT:

Background

Synaptogenesis is essential in forming new neurocircuits during development, and this is mediated in part by astrocyte-released thrombospondins (TSPs) and activation of their neuronal receptor, α2δ-1. Here, we show that this developmental synaptogenic mechanism is utilized during cocaine experience to induce spinogenesis and the generation of AMPA receptor-silent glutamatergic synapses in the adult nucleus accumbens shell (NAcSh).

Methods

Using multidisciplinary approaches including astrocyte Ca²⁺ imaging, genetic mouse lines, viral-mediated gene transfer, and operant behavioral procedures, we monitor the response of NAcSh astrocytes to cocaine administration and examine the role of astrocytic TSP-α2δ-1 signaling in cocaine-induced silent synapse genera

SUBMITTER: Wang J 

PROVIDER: S-EPMC7854999 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature