Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.

ABSTRACT: Cell sorting is a powerful tool in basic research and therapeutic enrichment. However, common cell sorting methods, such as fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) have significant limitations, such as generally low cell yields or restriction to binary separation, respectively. To address these limitations, we developed a two-step cell sorting method called mass-added density centrifugation (MADC) to enable nonbinary separation of large cell numbers based on surface protein levels. In the first MADC step (mass-adding), antibody-directed massive microparticles bind target surface proteins to modulate single-cell density proportionally to target protein level. Second, microparticle-laden cells are subjected to discontinuous density gradient centr

SUBMITTER: Sarnik SA 

PROVIDER: S-EPMC7855113 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature